Coding

Part:BBa_K1321365:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)


CBDcenA with linker fused to sfGFP driven by LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

The LacI vector was fused to the protein fusion using the Xba and SpeI site.

Source

CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part came from BBa_J04500.

References